September 10, 2025 — Awarded in 2019, the NSF EPSCoR RII Track-1 Maine-eDNA project has reached the conclusion of its NSF funding. The program, which investigated Maine’s coast, inland lakes and the waterways in between, championed environmental DNA (eDNA) as a powerful and cost-effective approach to monitoring an environment as small as a stream to as large as Maine’s coastline. Maine-eDNA Co-PI Michael Kinnison, University of Maine professor of evolutionary applications remarked, “The real power that we were seeing for eDNA is being able to look at the biology of the Maine coast at not just local but by very large scales that span habitats. That sort of snapshot of the coastal system is broadly something that’s really hard to get a handle on with other approaches.”
Bigelow Laboratory for Ocean Sciences Senior Research Scientist and Geomicrobiologist David Emerson served as a Co-PI for Maine-eDNA and authored the white paper that developed into the project’s proposal. “There are three things that really excite me about eDNA. Scientifically, it is the opportunity to study all organisms, from microbes to whales, within an ecosystem to see how they interconnect; collaboratively, it is the opportunity to work together with researchers from many different disciplines using a common language, DNA, and practically, it is the opportunity to develop an important new tool for ecosystem management and sustainability,” explained Emerson.
This genetic tool leverages the DNA shed by organisms in their environment. Researchers take a sample from the environment, in the case of Maine-eDNA as little as a liter of water, and, depending on method, identify the likely presence of a specific species or range of species in the vicinity. While like any technology there are tradeoffs, this allows researchers to accurately detect species presence in a relatively unobtrusive manner that does not rely on visual identification.
Researchers across Maine saw potential in the technology. “We saw an opportunity to push an emerging technology forward, become a leader in the field and benefit the lives of Mainers,” explained Kody Varahramyan, Maine-eDNA PI and UMaine Vice President for Research and Graduate Studies. “Maine-eDNA’s achievements exemplify the power of partnership and collaboration.”
eDNA technology has developed quickly over the past decade. When the original proposal for Maine-eDNA was in its infancy, researchers were exploring the capabilities of eDNA and interested in finding more applications, but the depth of real-world applications was limited. Fast-forward to 2024 and the President’s Office of Science and Technology Policy released their National Aquatic Environmental DNA Strategy which directs government offices to invest resources in the technology. During the interim years it was projects like Maine-eDNA that pushed the technology forward by improving methodologies, standardizing approaches, making data accessible to others, and pushing the scope of application into new areas.
Maine in many ways was a perfect testing ground as the technology offered a way for researchers to survey the vast expanse of Maine’s waters over the course of several years at a fraction of the price presented by other approaches. A survey of this size also demands the standardization and ground truthing the technology needed. Maybe most importantly, the project put eDNA technology in the hands of students, researchers, resource managers, businesses and other stakeholders through outreach and collaboration.